Doi T, Takemura S, Onodera H, Ueda M, Deguchi M, Nakahara R, Ichio N, Nakanishi S, Seto N, Yanagida K, Kondo M
First Department of Internal Medicine, Kyoto Prefectural University of Medicine.
Arerugi. 1997 Nov;46(11):1108-13.
One of the anaphylatoxins, C5a, is known to increase the expression of the complement receptors, CR1 and CR3, on PMNs which play important roles in the phagocytosis. We measured the expression of these receptors before and after the stimulation with C5a and C5a-receptors (C5aR) on PMNs in patients with systemic lupus erythematosus (SLE). PMNs from 16 patients and 11 normal controls were tested. All the patients with SLE were administered with prednisolone orally and were in the inactive stage. The CR1 expression in SLE was significantly weak (p < 0.01) before and after stimulation with 4.55 nM (50 micrograms/ml) of C5a. There was no significant difference of CR3 expression before stimulation. However, after the stimulation with C5a, the increase of CR3 on PMNs from SLE was significantly small (p < 0.01). C5aR on PMNs showed no difference between the two groups. However, the expression of C5aR was significantly suppressed in patients treated with a high dosage of prednisolone (> = 10 mg/day) compared to those with a low dosage of prednisolone (< 10 mg/day). There was no significant difference of CR1 and CR3 expression between these groups. It is concluded that the increase of CR1 and CR3 on PMNs by C5a in small in SLE, of which impaired increase is not due to C5aR on PMNs, and that the expression of C5aR is suppressed by prednisolone.
过敏毒素之一C5a已知可增加中性粒细胞上补体受体CR1和CR3的表达，这些受体在吞噬作用中起重要作用。我们测量了系统性红斑狼疮（SLE）患者中性粒细胞在C5a和C5a受体（C5aR）刺激前后这些受体的表达。对16例患者和11名正常对照者的中性粒细胞进行了检测。所有SLE患者均口服泼尼松龙且处于疾病非活动期。在用4.55 nM（50微克/毫升）C5a刺激前后，SLE患者的CR1表达均显著减弱（p<0.01）。刺激前CR3表达无显著差异。然而，在用C5a刺激后，SLE患者中性粒细胞上CR3的增加显著较小（p<0.01）。两组中性粒细胞上的C5aR无差异。然而，与低剂量泼尼松龙（<10毫克/天）治疗的患者相比，高剂量泼尼松龙（>=10毫克/天）治疗的患者C5aR表达显著受到抑制。这些组之间CR1和CR3表达无显著差异。结论是，在SLE中，C5a诱导的中性粒细胞上CR1和CR3的增加较小，其增加受损并非由于中性粒细胞上的C5aR，并且泼尼松龙可抑制C5aR的表达。
