Parinaud J, Le Lannou D, Vieitez G, Griveau J F, Milhet P, Richoilley G
Laboratoire FIV, CHU La Grave, Toulouse, France.
Hum Reprod. 1997 Nov;12(11):2434-6. doi: 10.1093/humrep/12.11.2434.
Oxygen radical generation is known to be detrimental to sperm function, especially motility, through the lipid peroxidation of the membranes. Generation of reactive oxygen species can be induced by leukocyte contamination, sperm centrifugation and the presence of abnormal spermatozoa with excess residual cytoplasm. This study aims to evaluate the effect on sperm motility of incubation in an antioxidant-containing solution, during liquefaction and centrifugation. Thirty semen samples were each divided into two equal parts: one mixed with Tyrode's solution, the other with a salt solution containing antioxidants (Sperm-Fit; Ellios Bio-Media, Paris, France). All the procedures were identical in the two groups. The ratio of leukocytes to spermatozoa was significantly correlated with the motility after liquefaction and after a 24 h incubation in routine in-vitro fertilization (IVF) medium and with the number of motile spermatozoa recovered after Percoll preparation. Moreover, when this ratio was > or = 0.2, all motility parameters were lowered. Incubation with Sperm-Fit allowed a higher percentage of motility after Percoll preparation when the ratio was > or = 0.2 (48 +/- 5% versus 41 +/- 6% for Sperm-Fit and Tyrode's solution respectively; P < 0.05) and a greater number of motile spermatozoa recovered after Percoll preparation, whatever the ratio (3.2 +/- 1.0 x 10(6) versus 2.4 +/- 0.7 x 10(6) for Sperm-Fit and Tyrode's solution respectively when ratio > or = 0.2; 18.1 +/- 3.4 x 10(6) versus 14.4 +/- 2.9 x 10(6) for Sperm-Fit and Tyrode's solution respectively when ratio < 0.2; P < 0.05). These results show that incubation with antioxidants during liquefaction and centrifugation increases recovery of motile spermatozoa.
众所周知，氧自由基的产生会通过细胞膜的脂质过氧化作用对精子功能，尤其是运动能力造成损害。活性氧的产生可由白细胞污染、精子离心以及存在过多残余细胞质的异常精子所诱导。本研究旨在评估在液化和离心过程中，于含抗氧化剂的溶液中孵育对精子运动能力的影响。30份精液样本各被分成两个相等的部分：一份与台氏液混合，另一份与含抗氧化剂的盐溶液（Sperm-Fit；法国巴黎埃利奥斯生物介质公司）混合。两组的所有操作均相同。在常规体外受精（IVF）培养基中液化后以及孵育24小时后的白细胞与精子比例，与珀可（Percoll）制备后回收的活动精子数量显著相关。此外，当该比例≥0.2时，所有运动参数均降低。当比例≥0.2时，用Sperm-Fit孵育使得珀可制备后的运动能力百分比更高（Sperm-Fit组为48±5%，台氏液组为41±6%；P<0.05），并且无论比例如何，珀可制备后回收的活动精子数量更多（当比例≥0.2时，Sperm-Fit组为3.2±1.0×10⁶，台氏液组为2.4±0.7×10⁶；当比例<0.2时，Sperm-Fit组为18.1±3.4×10⁶，台氏液组为14.4±2.9×10⁶；P<0.05）。这些结果表明，在液化和离心过程中用抗氧化剂孵育可增加活动精子的回收率。
