Kang M R, Cho Y K, Chun J, Kim Y B, Lee I, Lee H J, Kim S H, Kim Y K, Yoon K, Yang J M, Kim J M, Shin Y O, Kang C, Lee J S, Choi K W, Kim D G, Fitch W M, Kim S
Institute for Molecular Biology and Genetics and Medical College, Seoul National University, Korea.
J Acquir Immune Defic Syndr Hum Retrovirol. 1998 Jan 1;17(1):58-68. doi: 10.1097/00042560-199801010-00009.
To study the genetic variation of the HIV-1 strains prevalent in South Korea, we analyzed the nef sequences derived from 46 HIV-1-positive individuals living in various geographic regions in Korea. Phylogenetic analysis revealed four subtypes of HIV-1: A (3 patients), B (41 patients), D (1 patient), and a type that could not be clearly classified to any known subtype (1 patient). Thirty-five of the 41 Korean subtype B isolates formed a distinct monophyletic clade that is not related to any of the international sequences from the Los Alamos Database or GenBank as of June 1997. Indeed, the presence of unique conserved sequences was identified among the Korean isolates in this Korean subtype B group. The variations in the nucleotide sequences of a majority (32 of 35) subtype B samples within the Korean clade were 1.9% to 8.8%, and amino acid sequences varied from 3.9% to 15.5%. These results suggest that HIV-1 strains currently present in South Korea might have originated from a few sources or might be developing through a certain selective pressure. This is the first report on the molecular nature of the HIV-1 infection present in South Korea.
为研究韩国流行的HIV-1毒株的基因变异情况，我们分析了来自韩国不同地理区域的46名HIV-1阳性个体的nef序列。系统发育分析揭示了HIV-1的四种亚型：A（3例患者）、B（41例患者）、D（1例患者），以及一种无法明确归类到任何已知亚型的类型（1例患者）。41例韩国B亚型分离株中的35株形成了一个独特的单系分支，该分支与截至1997年6月洛斯阿拉莫斯数据库或GenBank中的任何国际序列均无关联。实际上，在这个韩国B亚型组的韩国分离株中鉴定出了独特的保守序列。韩国分支内大多数（35株中的32株）B亚型样本的核苷酸序列变异为1.9%至8.8%，氨基酸序列变异为3.9%至15.5%。这些结果表明，目前韩国存在的HIV-1毒株可能起源于少数几个来源，或者可能是在某种选择压力下发展而来。这是关于韩国HIV-1感染分子特性的首次报告。
