Inagaki T, Schoenwolf G C, Walker M L
Department of Neurobiology and Anatomy, University of Utah School of Medicine and Primary Children's Medical Center, Salt Lake City 84132, USA.
Pediatr Neurosurg. 1997 Apr;26(4):185-9. doi: 10.1159/000121188.
On the basis of experimental studies on mouse embryos in which spina bifida development of the brain ventricular system. Mouse embryos were exposed and surgically treated at gestation day 12 to produce spina bifida aperta, and operated embryos were cultured exo utero in the peritoneal cavity of the pregnant dam. Embryos were harvested at gestation day 14. Open neural tube defects resulted in 7 out of 10 embryos surviving surgery. Histological examination revealed that the sizes of ventricles in both the supra- and infratentorial compartments were greatly reduced in embryos with spina bifida aperta compared with control embryos. This result supports the hypothesis that leakage of cerebrospinal fluid through the opening of the caudal spine may be a major cause of brain abnormalities in patients with myelomeningocele.
基于对小鼠胚胎的实验研究，该研究涉及脊柱裂时脑室系统的发育情况。在妊娠第12天对小鼠胚胎进行暴露和手术处理以产生开放性脊柱裂，然后将接受手术的胚胎在怀孕母鼠的腹腔内进行体外培养。在妊娠第14天收获胚胎。10个胚胎中有7个在手术后存活，出现开放性神经管缺陷。组织学检查显示，与对照胚胎相比，开放性脊柱裂胚胎的幕上和幕下脑室大小均显著减小。这一结果支持了这样的假说，即脑脊液通过尾椎开口渗漏可能是脊髓脊膜膨出患者脑部异常的主要原因。
