Sobol C V, Nesterov V P
Sechenov Institute of Evolutionary Physiology and Biochemistry, RAS, St. Petersburg, Russia.
Gen Physiol Biophys. 1997 Jun;16(2):189-92.
Vascular smooth muscle of the frog was skinned by 0.2 mg/ml saponin for 20 mm. The skinned preparations activated by Ca2+, at 2 mmol/l Mg2+ and MgATP2-, gave half maximal force at Ca2+ = (1.32 +/- 0.07) x 10(-6) mol/l and maximal force at Ca2+ = 3.16 x 10(-5) mol/l (35 +/- 4% of the maximal contraction induced by KCl in living smooth muscles). The Hill coefficient was 1.51 +/- 0.11. Thus, the skinned vascular smooth muscle of the frog has Ca2+ sensitivity similar to that of various preparations from a variety of muscle types; the slope of the tension-pCa curve obtained was similar to that typical of mammalian smooth muscles.
用0.2毫克/毫升的皂角苷对青蛙的血管平滑肌进行去皮处理20分钟。在2毫摩尔/升的Mg2+和MgATP2-存在下，由Ca2+激活的去皮制剂，在Ca2+ = (1.32 +/- 0.07) x 10(-6) 摩尔/升时产生半数最大力，在Ca2+ = 3.16 x 10(-5) 摩尔/升时产生最大力（占活平滑肌中KCl诱导的最大收缩的35 +/- 4%）。希尔系数为1.51 +/- 0.11。因此，青蛙的去皮血管平滑肌具有与各种肌肉类型的不同制剂相似的Ca2+敏感性；所获得的张力-pCa曲线的斜率与哺乳动物平滑肌的典型斜率相似。
