Quigley D I, McDougall J, Darland T, Zhang G, Ronnekliev O, Grandy D K, Allen R G
Vollum Institute for Advanced Biomedical Research, Oregon Health Sciences University, Portland 97201, USA.
Peptides. 1998;19(1):133-9. doi: 10.1016/s0196-9781(97)00268-4.
In order to investigate the processing of OFQ containing peptides in the hypothalamus we have developed a sensitive and quantitative radioimmunoassay for OFQ. We fractionated rodent and monkey hypothalamic extracts by reversed-phase high performance liquid chromatography and found that the extracts contained multiple peaks of OFQ immunoreactivity with the major peak co-eluting with synthetic OFQ1-17. Mouse hypothalamic extracts were also fractionated by SDS-PAGE to determine the apparent molecular weights of molecules containing the OFQ peptide. Multiple peaks of OFQ immunoreactivity, ranging in size from approximately 1 to 30 kilodaltons, were detected by this method. These results suggest that OFQ1-17 is processed to smaller peptides in mouse and monkey hypothalamic neurons.
为了研究下丘脑内含孤啡肽（OFQ）的肽段的加工过程，我们开发了一种灵敏且定量的OFQ放射免疫分析法。我们通过反相高效液相色谱对啮齿动物和猴的下丘脑提取物进行了分离，发现提取物中含有多个OFQ免疫反应性峰，其中主要峰与合成的OFQ1-17共洗脱。还通过SDS-PAGE对小鼠下丘脑提取物进行了分离，以确定含OFQ肽的分子的表观分子量。通过该方法检测到了多个OFQ免疫反应性峰，大小范围约为1至30千道尔顿。这些结果表明，OFQ1-17在小鼠和猴的下丘脑神经元中被加工成较小的肽段。
