Lee E, Kang S G, Kehrli M E
Department of Microbiology, Immunology, and Preventive Medicine, Iowa State University Ames 50010, USA.
Vet Immunol Immunopathol. 1997 Oct 6;59(1-2):121-9. doi: 10.1016/s0165-2427(97)00051-2.
Intercellular adhesion molecule-1 (ICAM-1) is an inducible glycoprotein that interacts with the leukocyte beta 2-integrins, LFA-1 and Mac-1. We have isolated and analyzed a cDNA clone coding for the putative bovine ICAM-1 gene and compared it with known comparative sequences from other species as well as bovine ICAM-3. The 3398-bp bovine ICAM-1 cDNA sequence codes for 535 amino acids and shows 57% homology with human ICAM-1 and 47% homology with bovine ICAM-3 at the amino acid levels. The predicted number and positions of cysteine residues in bovine ICAM-1 are all conserved among species including bovine ICAM-3. It has two arginine-glycine-aspartate (RGD) sites in the extracellular region and a serine residue in the cytoplasmic tail. Northern blot results show that the bovine ICAM-1 gene is expressed in stimulated leukocytes whereas bovine ICAM-3 is expressed predominantly in resting neutrophils.
细胞间黏附分子-1（ICAM-1）是一种可诱导的糖蛋白，它能与白细胞β2-整合素LFA-1和Mac-1相互作用。我们已经分离并分析了一个编码假定牛ICAM-1基因的cDNA克隆，并将其与来自其他物种的已知比较序列以及牛ICAM-3进行了比较。3398个碱基对的牛ICAM-1 cDNA序列编码535个氨基酸，在氨基酸水平上与人ICAM-1的同源性为57%，与牛ICAM-3的同源性为47%。牛ICAM-1中半胱氨酸残基的预测数量和位置在包括牛ICAM-3在内的物种中都是保守的。它在细胞外区域有两个精氨酸-甘氨酸-天冬氨酸（RGD）位点，在细胞质尾部有一个丝氨酸残基。Northern印迹结果表明，牛ICAM-1基因在受刺激的白细胞中表达，而牛ICAM-3主要在静止的中性粒细胞中表达。
