Ohyashiki K, Ohyashiki J H, Yahata N, Toyama K
First Department of Internal Medicine, Tokyo Medical College.
Rinsho Byori. 1997 Dec;45(12):1133-41.
Telomerase is a ribonuclear protein that is detected in more than 90% of primary cancer tissues using a telomeric repeat amplification protocol (TRAP) assay, thus, telomerase is considered to be a novel marker for cancer. Telomerase activity is not detectable in somatic cells, except for hematopoietic cells and cryptic cells in the intestine and hair follicles, thus, quantitation of telomerase is important to delineate clinical implication of telomerase activity. We have developed semiquantitative fluorescence-based TRAP assay. Moreover, we also developed an in situ TRAP assay that detects telomerase activity at the cellular level. Thus, using these TRAP assays, we are able to detect telomerase activity in various kinds of extracts or cytological specimens and therefore these applications may have additive information in the early detection of cancer and monitoring disease condition.
端粒酶是一种核糖核蛋白，使用端粒重复序列扩增法（TRAP）检测发现，超过90%的原发性癌组织中可检测到端粒酶，因此，端粒酶被认为是一种新型癌症标志物。除造血细胞、肠道隐窝细胞和毛囊细胞外，体细胞中检测不到端粒酶活性，因此，端粒酶定量对于阐明端粒酶活性的临床意义很重要。我们开发了基于荧光的半定量TRAP检测法。此外，我们还开发了一种原位TRAP检测法，可在细胞水平检测端粒酶活性。因此，使用这些TRAP检测法，我们能够在各种提取物或细胞学标本中检测端粒酶活性，因此这些应用在癌症早期检测和疾病状况监测中可能具有补充信息。
