Hidaka H, Tozuka M, Yamauchi K, Sugano M, Nakabayashi T, Katsuyama T
Central Clinical Laboratories, Shinshu University Hospital, Matsumoto.
Rinsho Byori. 1997 Dec;45(12):1172-6.
Distribution of apolipoprotein(apo) E4 and E3 in lipoproteins of serum with apoE4/E3 phenotype was analyzed. ApoE was eluted in two major peaks by gel chromatography; peak 1 and 2 corresponding to very- and intermediate-low density lipoprotein (VLDL + IDL) and high density lipoprotein2 (HDL2), respectively. ApoE in peak 1 (VLDL + IDL) consisted of monomers of 34 kDa, complexes with a high molecular weight (apoEs) of 100 kDa and with a small amount of apoE-AII complexes weighing 43 kDa. In contrast, apoE in peak 2 (HDL2) was composed mainly of apoE-AII complexes and apoEs complexes, and a small amount of monomers. Both apoE3 and E4 isoforms were detected in these peaks; E4 was more predominant in peak 1 while E3 was more predominant in peak 2. These findings suggest that different distributions of apoE3 and E4 in lipoprotein particles.
分析了具有载脂蛋白E4/ E3表型的血清脂蛋白中载脂蛋白（apo）E4和E3的分布情况。通过凝胶色谱法，载脂蛋白E以两个主要峰洗脱；峰1和峰2分别对应极低密度脂蛋白和中间密度脂蛋白（VLDL + IDL）以及高密度脂蛋白2（HDL2）。峰1（VLDL + IDL）中的载脂蛋白E由34 kDa的单体、100 kDa的高分子量复合物（apoEs）以及少量43 kDa的载脂蛋白E - AII复合物组成。相比之下，峰2（HDL2）中的载脂蛋白E主要由载脂蛋白E - AII复合物和apoEs复合物以及少量单体组成。在这些峰中均检测到了载脂蛋白E3和E4异构体；E4在峰1中更占优势，而E3在峰2中更占优势。这些发现表明载脂蛋白E3和E4在脂蛋白颗粒中的分布不同。
