Sulakvelidze I, Conway M, Evans S, Stetsko P I, Djuric V, Dolovich J
Department of Pediatrics, McMaster University, Hamilton, Ontario, Canada.
Am J Rhinol. 1997 Nov-Dec;11(6):435-41. doi: 10.2500/105065897780914965.
Ten patients with perennial allergic rhinitis and 10 healthy subjects were studied to determine most discriminative nasal irrigation fluid marker(s) and to compare samples that were collected at baseline and over a 1-hour period, every 15 minutes. The latter were pooled and designated 1-hour sample. In the nasal irrigation we investigated the following inflammatory cells and soluble mediators: eosinophils, neutrophils, granulocyte-macrophage colony-stimulating factor, interleukin-4, interleukin-6, interleukin-8, ECP, EPX, MPO, leukotriene C4, leukotriene B4, prostaglandin E2, tryptase and fibrinogen. Patients with PAR were then treated for 2 weeks with the topical nasal steroid. The only marker that discriminated patients with perennial allergic rhinitis and healthy subjects was eosinophil count (EO%): correspondingly 14.01 +/- 5.8 and 0.18 +/- 0.09, (M +/- SD). Difference between the studied groups did not depend on the time of irrigation, baseline or 1-hour. EO% was also the only marker of a clinically successful treatment with the nasal steroid, 14.01 +/- 5.8 and 0.87 +/- 0.4, before and after treatment respectively. We conclude that EO% is the most sensitive inflammatory marker of perennial allergic rhinitis, and that baseline nasal irrigation can be used to study nasal mucosal inflammation.
对10例常年性变应性鼻炎患者和10名健康受试者进行研究，以确定最具鉴别性的鼻腔灌洗液标志物，并比较在基线时以及在1小时内每隔15分钟采集的样本。将后者合并并指定为1小时样本。在鼻腔灌洗中，我们研究了以下炎性细胞和可溶性介质：嗜酸性粒细胞、中性粒细胞、粒细胞-巨噬细胞集落刺激因子、白细胞介素-4、白细胞介素-6、白细胞介素-8、嗜酸性粒细胞阳离子蛋白（ECP）、嗜酸性粒细胞过氧化物酶（EPX）、髓过氧化物酶（MPO）、白三烯C4、白三烯B4、前列腺素E2、类胰蛋白酶和纤维蛋白原。然后，常年性变应性鼻炎患者接受局部鼻用类固醇治疗2周。区分常年性变应性鼻炎患者和健康受试者的唯一标志物是嗜酸性粒细胞计数（EO%）：相应地分别为14.01±5.8和0.18±0.09，（均值±标准差）。研究组之间的差异不取决于灌洗时间，无论是基线时还是1小时时。EO%也是鼻用类固醇临床治疗成功的唯一标志物，治疗前后分别为14.01±5.8和0.87±0.4。我们得出结论，EO%是常年性变应性鼻炎最敏感的炎性标志物，并且基线鼻腔灌洗可用于研究鼻黏膜炎症。
