Cho J J, Vliagoftis H, Rumsaeng V, Metcalfe D D, Oh C K
Department of Pediatrics, Harbor-UCLA Medical Center, Torrance 90502, USA.
Biochem Biophys Res Commun. 1998 Jan 6;242(1):226-30. doi: 10.1006/bbrc.1997.7644.
Mast cells play an important role in allergic inflammation by releasing inducible proinflammatory cytokines. While many inducible genes have been identified, we hypothesized that a significant number remain to be identified. We thus constructed an activation-specific mast cell subtraction library to establish a profile of induced genes in mast cells following allergic stimulation. To date, we have sequenced 150 cDNA clones. Among them, we have isolated 22 known genes whose expression has not been reported in mast cells, and an additional 26 cDNA clones which do not have significant homology to known genes in the Genbank database. We next selected 10 cDNA clones with strong signals by differential plaque hybridization. Of these cDNA clones, five genes were induced in mast cells upon Fc epsilon RI-mediated stimulation. They are cofilin, annexinVI, interferon (IFN)-beta, serglycin, and a novel inducible mast cell (IMC) gene, IMC-415. Characterization and relevant studies of this novel gene and other inducible known genes in mast cells will provide insight into the functions of mast cells in mammalian biology.
肥大细胞通过释放诱导性促炎细胞因子在过敏性炎症中发挥重要作用。虽然已经鉴定出许多诱导性基因，但我们推测仍有大量基因有待鉴定。因此，我们构建了一个激活特异性肥大细胞消减文库，以建立过敏性刺激后肥大细胞中诱导基因的图谱。迄今为止，我们已经对150个cDNA克隆进行了测序。其中，我们分离出了22个在肥大细胞中尚未报道过表达的已知基因，以及另外26个与Genbank数据库中的已知基因没有显著同源性的cDNA克隆。接下来，我们通过差异噬菌斑杂交选择了10个信号强烈的cDNA克隆。在这些cDNA克隆中，有5个基因在FcεRI介导的刺激下在肥大细胞中被诱导。它们是丝切蛋白、膜联蛋白VI、干扰素（IFN）-β、丝甘蛋白聚糖，以及一个新的诱导性肥大细胞（IMC）基因IMC-415。对这个新基因以及肥大细胞中其他诱导性已知基因的表征和相关研究将为深入了解肥大细胞在哺乳动物生物学中的功能提供线索。
