Hitomi S, Su W D, Hong L J, Ohtsuka A, Murakami T
Department of Anatomy, Okayama University Medical School, Japan.
Acta Med Okayama. 1997 Dec;51(6):295-9. doi: 10.18926/AMO/30773.
Sections of the visual cortex of newborn (1-4 weeks after birth) and adult cats were stained with cationic iron colloid, aldehyde fuchsin or lectins (lectin Vicia villosa, soybean and Wisteria floribunda agglutinins). Many neurons in the adult cat visual cortex contained perineuronal sulfated proteoglycans detectable with cationic iron colloid and aldehyde fuchsin, or cell surface glycoproteins reactive to lectins. Double staining indicated that some of the lectin-labeled neurons were not stained with cationic iron colloid, and also that some of the cationic iron colloid-stained neurons were not labeled with lectins. The perineuronal sulfated proteoglycans and cell surface glycoproteins developed 3 weeks after birth. In the newborn cats 1-2 weeks after birth, no neurons were reactive to cationic iron colloid, aldehyde fuchsin or lectins. In the newborn cats 3-4 weeks after birth, it was clearly observed that the cytoplasm of the glial cells closely associated with the neurons containing the perineuronal sulfated proteoglycans showed an intense reaction to cationic iron colloid and aldehyde fuchsin, and that the Golgi complexes of the neurons with cell surface glycoproteins were intensely labeled with lectins. These findings suggest that the perineuronal sulfated proteoglycans are derived from the associated glial cells, and that the cell surface glycoproteins are produced by the associated nerve cells.
对新生猫（出生后1 - 4周）和成年猫的视觉皮层切片用阳离子铁胶体、醛复红或凝集素（野豌豆凝集素、大豆凝集素和紫藤凝集素）进行染色。成年猫视觉皮层中的许多神经元含有可被阳离子铁胶体和醛复红检测到的神经元周围硫酸化蛋白聚糖，或对凝集素反应性的细胞表面糖蛋白。双重染色表明，一些被凝集素标记的神经元未被阳离子铁胶体染色，而且一些被阳离子铁胶体染色的神经元也未被凝集素标记。神经元周围硫酸化蛋白聚糖和细胞表面糖蛋白在出生后3周发育形成。在出生后1 - 2周的新生猫中，没有神经元对阳离子铁胶体、醛复红或凝集素产生反应。在出生后3 - 4周的新生猫中，可以清楚地观察到，与含有神经元周围硫酸化蛋白聚糖的神经元紧密相关的神经胶质细胞的细胞质对阳离子铁胶体和醛复红呈现强烈反应，并且具有细胞表面糖蛋白的神经元的高尔基体复合体被凝集素强烈标记。这些发现表明，神经元周围硫酸化蛋白聚糖源自相关的神经胶质细胞，而细胞表面糖蛋白由相关的神经细胞产生。
