The effects of ciliary neurotrophic factor on murine spinal cord neurons subjected to dendrite transection injury.

Ciliary neurotrophic factor (CNTF) has been found to increase neuronal survival during development and after axotomy. The present study tested the effects of CNTF on lesioned and uninjured mouse spinal cord (SC) neurons grown in tissue culture. An initial toxicity study found that a 24-72 h exposure of SC cultures to concentrations of CNTF above 1000 ng/ml caused stress and death of unlesioned neurons and glia. Pre-selected SC neurons were then subjected to transection of a primary dendrite 100 microns from the edge of the perikaryon (approximately 50% average survival at 24 h). Application of CNTF at concentrations ranging from 0.5 to 1000 ng/ml immediately after lesioning had no statistically significant effects on SC neuron survival 24 h after dendrotomy. Separation of control (no CNTF) and CNTF-treated cells into groups of putative alpha-motor (multipolar with somal diameters > or = 25 microns) and non-alpha-motor neurons (< 25 microns somal diameters) also failed to reveal any significant differences in survival. The lack of protection by CNTF of lesioned SC neurons in mature (21-28 DIV) cultures may reflect a loss of sensitivity to CNTF that occurs with development. Alternatively, protection by CNTF may require co-factors or factors that are released from target or other cells after injury but that are not present in SC cultures.