Immunolocation of mitochondria-rich cells in epidermis of the common toad, Bufo bufo L.

The mitochondria-rich cells (MRCs) in adult toad epidermis have an important osmoregulatory function, being specialized for chloride transport. Immunostaining with monoclonal antibodies against human keratins was used to compare the keratin expressed by MRCs with that of principal cells (PCs). By the indirect immunoperoxidase technique, keratin epitopes in vertical cryosections (4-6 microns) of toad skin were labelled and examined by light microscopy. MRCs were stained selectively by the antibodies 6B10 (anti-keratin 4) and RCK102 (anti-keratins 5 and 8). In contrast, MRCs were not detected by the oligospecific MoAb K 8.12 that recognized the intermediate PCs, but not the basal ones. The polyspecific antibody LP34 stained both the MRCs and the basal PCs, whereas the antibody RCK107 (anti-keratin 14) stained MRCs intensely and part of the basal PCs more weakly. These immunoreactions indicate that the MRCs and the intermediate PCs express amphibian equivalents to the human keratins 4/5 and 13/15, respectively. Notably, MRCs were detected in all epidermal layers except stratum corneum. Presumably, the MRCs originate in the basal layer and retain their expression of an amphibian equivalent to the human keratin pair 4/5 + 14 throughout epidermis.