Yamakawa Y, Shiraishi H, Yamakawa M
Psychiatry Service, University of Tsukuba Hospital, Ibaraki, Japan.
Comp Biochem Physiol B Biochem Mol Biol. 1997 Oct;118(2):457-61. doi: 10.1016/s0305-0491(97)00172-7.
Nitric oxide synthase (NOS) activity in the chicken brain was examined, and higher activity was detected in the cerebellum. The NOS activity in the cerebellum was significantly inhibited in vitro by W-5, W-7 and trifluoperazine, and recovery of the activity was observed by the addition of calmodulin, suggesting that NOS is calmodulin dependent. Moreover, effects of EGTA and Ca2+ on NOS activity were also examined, and the results indicated that the enzyme is Ca2+ dependent. In addition, cofactors such as NADPH, flavin adenine dinucleotide (FAD) and flavin mononucleotide (FMN) were required for the full expression of NOS. The results suggest that chicken cerebellum NOS has similar properties to those of mammalian neural NOS.
对鸡脑内一氧化氮合酶（NOS）的活性进行了检测，结果发现在小脑中该酶活性较高。在体外，W - 5、W - 7和三氟拉嗪可显著抑制小脑中的NOS活性，而添加钙调蛋白后可观察到活性恢复，这表明NOS是钙调蛋白依赖性的。此外，还研究了乙二醇双（2-氨基乙基醚）四乙酸（EGTA）和钙离子对NOS活性的影响，结果表明该酶是钙离子依赖性的。另外，NOS的充分表达还需要烟酰胺腺嘌呤二核苷酸磷酸（NADPH）、黄素腺嘌呤二核苷酸（FAD）和黄素单核苷酸（FMN）等辅助因子。这些结果提示鸡小脑NOS与哺乳动物神经NOS具有相似的特性。
