Hildebrandt A, Sauer H W
Biochim Biophys Acta. 1976 Mar 17;425(3):316-21. doi: 10.1016/0005-2787(76)90258-6.
Two RNA polymerase activities were quantitatively solubilized in plasmodial homogenates from Physarum polycephalum by sonication at 0.5 M ammonium chloride concentration. The proportions of RNA polymerases A and B were determined by four different methods. Equal activity levels of both enzyme A and enzyme B were detected throughout the synchronous mitotic cycle of Physarum.
在0.5M氯化铵浓度下通过超声处理,从多头绒泡菌的原质团匀浆中定量溶解出两种RNA聚合酶活性。通过四种不同方法确定了RNA聚合酶A和B的比例。在多头绒泡菌的整个同步有丝分裂周期中,检测到酶A和酶B的活性水平相等。