苜蓿根瘤菌外切多聚半乳糖醛酸酶 ExoP 蛋白富含脯氨酸基序中的特定氨基酸替换导致低分子量琥珀酰聚糖产量增加，而高分子量琥珀酰聚糖产量减少。
Specific amino acid substitutions in the proline-rich motif of the Rhizobium meliloti ExoP protein result in enhanced production of low-molecular-weight succinoglycan at the expense of high-molecular-weight succinoglycan.
作者信息
Becker A, Pühler A
机构信息
Lehrsthul für Genetik, Fakultät für Biologie, Universität Bielefeld, Germany.
出版信息
J Bacteriol. 1998 Jan;180(2):395-9. doi: 10.1128/JB.180.2.395-399.1998.
Abstract
The production of the acidic exopolysaccharide succinoglycan (EPS I) by Rhizobium meliloti exoP* mutants expressing an ExoP protein lacking its C-terminal cytoplasmic domain and by mutants characterized by specific amino acid substitutions in the proline-rich motif (RX4PX2PX4SPKX9IXGXMXGXG) located from positions 443 to 476 of the ExoP protein was analyzed. The absence of the C-terminal cytoplasmic ExoP domain (positions 484 to 786) and the substitution of both arginine443 by isoleucine443 and proline457 by serine457 within the proline-rich motif resulted in enhanced production of low-molecular-weight (LMW) EPS I at the expense of high-molecular-weight (HMW) EPS I. The ratios of HMW to LMW EPS I of the wild type and mutant strains increased with osmolarity.
摘要
分析了表达缺少C端胞质结构域的ExoP蛋白的苜蓿根瘤菌exoP*突变体以及在ExoP蛋白443至476位富含脯氨酸基序（RX4PX2PX4SPKX9IXGXMXGXG）中有特定氨基酸取代的突变体产生酸性胞外多糖琥珀聚糖（EPS I）的情况。ExoP蛋白C端胞质结构域（484至786位）的缺失以及富含脯氨酸基序内精氨酸443被异亮氨酸443取代和脯氨酸457被丝氨酸457取代，导致低分子量（LMW）EPS I产量增加，而高分子量（HMW）EPS I产量降低。野生型和突变体菌株中HMW与LMW EPS I的比例随渗透压增加而升高。
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Specific amino acid substitutions in the proline-rich motif of the Rhizobium meliloti ExoP protein result in enhanced production of low-molecular-weight succinoglycan at the expense of high-molecular-weight succinoglycan.苜蓿根瘤菌外切多聚半乳糖醛酸酶 ExoP 蛋白富含脯氨酸基序中的特定氨基酸替换导致低分子量琥珀酰聚糖产量增加，而高分子量琥珀酰聚糖产量减少。
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