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可移动拟杆菌转座子Tn4555的转移起源与革兰氏阳性菌的一个质粒家族有关。

The transfer origin for Bacteroides mobilizable transposon Tn4555 is related to a plasmid family from gram-positive bacteria.

作者信息

Smith C J, Parker A C

机构信息

Department of Microbiology and Immunology, East Carolina University, Greenville, North Carolina 27858, USA.

出版信息

J Bacteriol. 1998 Jan;180(2):435-9. doi: 10.1128/JB.180.2.435-439.1998.

DOI:10.1128/JB.180.2.435-439.1998
PMID:9440538
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC106904/
Abstract

Conjugal transfer of Bacteroides mobilizable transposon Tn4555 was examined with an Escherichia coli-based assay system. It was shown that mobilization required the cis-acting oriT(Tn) region and that the Tn4555 mobA(Tn) gene and RK231 must be present in trans. With alkaline agarose gel electrophoresis and filter blot hybridizations, it was shown that at oriT(Tn) there was a site- and strand-specific cleavage event that was dependent on mobA(Tn). The 5' end of this cleavage site was mapped by primer extension, and the nucleotide sequence surrounding the site had homology to a family of oriT nick sites found in mobilizable plasmids of gram-positive bacteria. Removal of the nick site by deletion of 18 bp surrounding the site resulted in a significant loss of transfer activity.

摘要

利用基于大肠杆菌的检测系统研究了可移动转座子Tn4555在配偶体间的转移。结果表明，转移需要顺式作用的oriT(Tn)区域，并且Tn4555 mobA(Tn)基因和RK231必须反式存在。通过碱性琼脂糖凝胶电泳和滤膜杂交表明，在oriT(Tn)处存在一个依赖于mobA(Tn)的位点和链特异性切割事件。通过引物延伸确定了该切割位点的5'端，该位点周围的核苷酸序列与革兰氏阳性菌可移动质粒中发现的oriT切口位点家族具有同源性。通过缺失该位点周围18 bp的序列去除切口位点，导致转移活性显著丧失。

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