Saporta S, Borlongan C, Moore J, Mejia-Millan E, Jones S L, Bonness P, Randall T S, Allen R C, Freeman T B, Sanberg P R
Department of Anatomy, University of South Florida College of Medicine, Tampa 33612-4799, USA.
Cell Transplant. 1997 Nov-Dec;6(6):579-84. doi: 10.1177/096368979700600608.
The transplantation of tissue containing dopamine-producing cells into the mammalian central nervous system is an emerging treatment for Parkinson's disease, despite relatively poor survival of implanted tissue. Recent evidence has suggested that Cytodex microcarriers enhance the survival of dopaminergic rat chromaffin cells transplanted into the rat striatum in the absence of immunosuppression. The current study was undertaken to evaluate the survival of rat and human fetal ventral mesencephalic neurons (VM) implanted alone or after attachment to microcarriers in the striatum of rats without immunosuppression. Rat fetal VM neurons demonstrated enhanced survival in the rat striatum when transplanted on microcarriers, compared to their transplantation alone during the 3-mo period examined in the present study. Transplants of human fetal VM neurons on microcarriers also survived remarkably well in the rat striatum without systemic immunosuppression. In contrast, human fetal VM cells transplanted alone into the rat striatum did not survive without systemic immunosuppression. There was no evidence of TH fiber sprouting in the vicinity of any transplant site. These data indicated that Cytodex microcarriers provide enhanced survival of both rat allograft and human xenograft fetal mesencephalic cells in the rat striatum without the necessity of systemic immunosuppression, perhaps by inducing a unique neuron-glia environment.
将含有产生多巴胺细胞的组织移植到哺乳动物中枢神经系统中是帕金森病的一种新兴治疗方法，尽管植入组织的存活率相对较低。最近的证据表明，在没有免疫抑制的情况下，Cytodex微载体可提高移植到大鼠纹状体中的多巴胺能大鼠嗜铬细胞的存活率。本研究旨在评估单独植入或附着于微载体后的大鼠和人类胎儿腹侧中脑神经元（VM）在无免疫抑制的大鼠纹状体中的存活率。在本研究中检查的3个月期间，与单独移植相比，大鼠胎儿VM神经元在微载体上移植时在大鼠纹状体中的存活率提高。在没有全身免疫抑制的情况下，微载体上的人类胎儿VM神经元移植在大鼠纹状体中也存活得非常好。相比之下，单独移植到大鼠纹状体中的人类胎儿VM细胞在没有全身免疫抑制的情况下无法存活。在任何移植部位附近均未发现TH纤维发芽的证据。这些数据表明，Cytodex微载体可提高大鼠纹状体中大鼠同种异体移植和人类异种移植胎儿中脑细胞的存活率，而无需全身免疫抑制，这可能是通过诱导独特的神经元-神经胶质环境实现的。
