Helms S R, Pretlow T G, Bueschen A J, Lloyd K L, Murad T M
Cancer Res. 1976 Feb;36(2 Pt 1):481-6.
This report describes the separation of cells exhibiting histochemically demonstrable acid phosphatase from suspensions of cells obtained from human prostatic carcinomas by velocity sedimentation. In the unseparated suspensions of cells, 40.5 +/- 7.7% of nucleated cells contained histochemically evident acid phosphatase. After cells were separated by velocity sedimentation, 86.4 +/- 9.4% of the nucleated cells in the purest fractions exhibited histochemically demonstrable acid phosphatase activity. More than 95% of these cells excluded trypan blue. To our knowledge, this is the first report of a method for the separation of viable epithelial cells from human prostatic carcinomas.
本报告描述了通过速度沉降法从人前列腺癌细胞悬液中分离出具有组织化学可显示酸性磷酸酶的细胞。在未分离的细胞悬液中，40.5±7.7%的有核细胞含有组织化学上明显的酸性磷酸酶。通过速度沉降法分离细胞后，最纯级分中有86.4±9.4%的有核细胞表现出组织化学可显示的酸性磷酸酶活性。这些细胞中超过95%排斥台盼蓝。据我们所知，这是关于从人前列腺癌中分离活上皮细胞方法的首次报告。
