Akopov S B, Nikolaev L G, Ruzov A S, Sverdlov E D
Bioorg Khim. 1997 Sep;23(9):727-31.
Fifteen sequences belonging to the Chinese hamster genome were isolated from a library of sequences preferentially binding to the nuclear matrix (matrix attachment regions, MAR), sequenced, and characterized. Fourteen of the 15 sequences (> 90%) bound to the nuclear matrix with affinities 2.5-60 times higher than those of control DNA fragments containing no MARs. One clone displayed a considerable homology to the ORF1 region of the mouse LINE repeat. Such MARs within LINE repeats may considerably alter the activities of some genes and the transcription status of chromatin domains upon the LINE repeat propagation in the genome over the course of evolution.
从一个优先与核基质结合的序列文库（基质附着区域，MAR）中分离出15个属于中国仓鼠基因组的序列，进行测序并表征。15个序列中的14个（>90%）以比不含MAR的对照DNA片段高2.5至60倍的亲和力与核基质结合。一个克隆与小鼠LINE重复序列的ORF1区域显示出相当高的同源性。在进化过程中，LINE重复序列中的此类MAR可能会在LINE重复序列在基因组中传播时显著改变某些基因的活性和染色质结构域的转录状态。
