Albala J S, Thelen M P, Prange C, Fan W, Christensen M, Thompson L H, Lennon G G
Biology and Biotechnology Research Program, Lawrence Livermore National Laboratory, California 95441-0808, USA.
Genomics. 1997 Dec 15;46(3):476-9. doi: 10.1006/geno.1997.5062.
The highly conserved Saccharomyces cerevisiae RAD51 protein functions in both mitotic and meiotic homologous recombination and in double-strand break repair. Screening of the public cDNA sequence database for RAD51-like genes led to the identification of a partial sequence from a breast tissue library present in the I.M.A.G.E. (Integrated Molecular Analysis of Genes and their Expression) collection. An extended 1764-bp cDNA clone encoding an open reading frame of 350 amino acids was isolated. This clone showed significant amino acid identity with other human RAD51 homologs. The new homolog, named RAD51B, was mapped to human chromosome 14q23-q24.2 using a panel of human-hamster somatic cell hybrids and fluorescence in situ hybridization. Northern blot analysis demonstrated that RAD51B mRNA is widely expressed and most abundant in tissues active in recombination. Functions associated with known RAD51 homologs suggest a role for RAD51B in meiotic recombination and/or recombinational repair.
高度保守的酿酒酵母RAD51蛋白在有丝分裂和减数分裂同源重组以及双链断裂修复中均发挥作用。在公共cDNA序列数据库中筛选RAD51样基因，从而在I.M.A.G.E.（基因及其表达的综合分子分析）文库中的乳腺组织文库里鉴定出一段部分序列。分离出一个长度为1764 bp的延伸cDNA克隆，其编码一个由350个氨基酸组成的开放阅读框。该克隆与其他人RAD51同源物具有显著的氨基酸同一性。这个新的同源物被命名为RAD51B，利用一组人-仓鼠体细胞杂种和荧光原位杂交技术将其定位于人类染色体14q23-q24.2。Northern印迹分析表明，RAD51B mRNA广泛表达，且在活跃于重组的组织中最为丰富。与已知RAD51同源物相关的功能表明RAD51B在减数分裂重组和/或重组修复中发挥作用。
