Seki M, Yamaguchi K, Marumo H, Imai K
Hatano Research Institute, Food and Drug Safety Center, Laboratory of Toxicology, Kanagawa, Japan.
J Toxicol Sci. 1997 Dec;22(5):427-37. doi: 10.2131/jts.22.5_427.
Eighty Sprague-Dawley rats, consisting of 40 animals of each sex, were examined for age-related lesions after a 13-week food restriction. From 6 weeks of age, four groups of 10 male and 10 female rats each were fed normal pelleted chow, ad libitum for the control group and approximately 85%, 70% and 55% of feed consumption in the control group, for 13 weeks. Estrous cycles in females were examined during the 3rd through 12th week of the study, and hematological, blood biochemical and pathological examinations of all animals were carried out after the 13-week feeding. Estrous cycles were prolonged or stopped in most females of the 55% feeding group from the 7th week of restricted feeding and atrophy of the theca granulosa cells with decreased cytoplasmic lipid droplets was found in the ovaries at necropsy. In the males of the 55% feeding group, Leydig cell atrophy was found in the testes. The groups at 70% and 85% feeding showed no abnormality in hematological, blood biochemical and pathological observations. On the other hand, decreases in plasma lipid peroxide levels were noted in the 70 and 85% feeding groups. These results suggest that 70% and 85% feedings seem suitable for minimizing development of age-related changes without nutritional disorders.
80只Sprague-Dawley大鼠（雌雄各40只）在进行13周的食物限制后，接受了与年龄相关病变的检查。从6周龄开始，将四组大鼠（每组10只雄性和10只雌性）分别喂食正常颗粒饲料，对照组随意进食，而其他三组分别给予对照组饲料消耗量约85%、70%和55%的饲料，持续13周。在研究的第3周至第12周期间检查雌性大鼠的发情周期，并在13周喂食后对所有动物进行血液学、血液生化和病理学检查。在限制喂食第7周后，55%喂食组的大多数雌性大鼠发情周期延长或停止，尸检时发现卵巢中卵泡膜颗粒细胞萎缩，细胞质脂滴减少。在55%喂食组的雄性大鼠中，发现睾丸间质细胞萎缩。70%和85%喂食组在血液学、血液生化和病理学观察中未显示异常。另一方面，70%和85%喂食组的血浆脂质过氧化物水平有所下降。这些结果表明，70%和85%的喂食量似乎适合在不出现营养紊乱的情况下将与年龄相关变化的发展降至最低。
