Pérez del Castillo M M, Lostao M P, Barber A, Ponz F
Departamento de Fisiología y Nutrición, Universidad de Navarra, Pamplona, Spain.
Rev Esp Fisiol. 1997 Sep;53(3):281-7.
Some modifications to the method of PONZ et al. for in vivo intestinal absorption studies using an in situ perfused segment of small intestine, under anesthesia, are described. They improve its accuracy and applicability, especially when slowly absorbed substrates are used or when volume flux measurements are desired. Calculations for the absorbed substrate and net fluid volume change determinations are reported. Several aspects of the use of the method under a single pass or a recirculation perfusion system are discussed.
本文描述了对PONZ等人的方法所做的一些改进，该方法用于在麻醉状态下通过小肠原位灌注段进行体内肠道吸收研究。这些改进提高了该方法的准确性和适用性，尤其是在使用吸收缓慢的底物或需要测量体积通量时。报告了吸收底物的计算方法和净液体体积变化的测定方法。还讨论了在单程或再循环灌注系统下使用该方法的几个方面。
