Hafs H D
J Toxicol Environ Health. 1976 Mar;1(4):669-79. doi: 10.1080/15287397609529365.
Often in developing hormone assays, hormones that may interfere with the assay by cross-reaction are not available for testing the validity of the assay. For example, horse TSH was unavailable to test for cross-reaction in an LH radioimmunoassay (RIA). The authors devised an indirect means of accomplishing the same goal, and the evidence from the indirect test of cross-reaction was at least as persuasive as a direct test might have been. Other examples are given of experiments where extensive effort was devoted to validation of steroid RIA, but there were substantial quantitative differences in the results among experiments and among laboratories. Differences of this kind probably would be intolerable in an assay used to monitor hormone residues in food-producing animals.
在开发激素检测方法时，常因无法获得可能通过交叉反应干扰检测的激素，从而无法对检测方法的有效性进行测试。例如，在促黄体生成素放射免疫分析（RIA）中，无法获得马促甲状腺激素来检测交叉反应。作者设计了一种间接方法来实现相同的目标，交叉反应间接测试的证据至少与直接测试的证据一样有说服力。文中还给出了其他实验示例，这些实验在类固醇RIA验证方面投入了大量精力，但不同实验之间以及不同实验室之间的结果存在显著的定量差异。在用于监测食用动物激素残留的检测方法中，这种差异可能是无法接受的。
