Yajima Y, Hayakawa T, Hayashi Y
Department of Physiology, Hyogo College of Medicine, Japan.
Acta Otolaryngol Suppl. 1997;532:132-4.
Laryngeal motoneurons in the nucleus ambiguus were activated antidromically to ipsilateral recurrent nerve stimulation in urethane-chloralose anaesthetized rats. GABAA agonist muscimol and the antagonist bicuculline were applied separately or together with GABA iontophoretically with a multibarrel pipette glued with a recording electrode. The majority of neurons (119/155) which did not respond to the recurrent nerve stimulation exhibited activities mostly related to inspiration. Other 32 neurons were classified as laryngeal motoneurons, according to the criteria. Bicuculline application antagonized GABAA-mediated inhibition while muscimol or GABA reversed this effect significantly. Dose dependent changes were observed for administration of these GABAA-related agents. It can be concluded therefore that laryngeal motoneurons received GABAA-mediated inhibition.
在氨基甲酸乙酯-氯醛糖麻醉的大鼠中，疑核内的喉运动神经元在同侧喉返神经刺激时被逆向激活。使用与记录电极黏合的多管移液管，分别或联合离子电渗法施加GABAA激动剂蝇蕈醇和拮抗剂荷包牡丹碱以及GABA。大多数对喉返神经刺激无反应的神经元（119/155）表现出主要与吸气相关的活动。根据标准，另外32个神经元被归类为喉运动神经元。施加荷包牡丹碱可拮抗GABAA介导的抑制作用，而蝇蕈醇或GABA可显著逆转这种作用。观察到这些与GABAA相关的药物给药存在剂量依赖性变化。因此可以得出结论，喉运动神经元受到GABAA介导的抑制。
