福寿螺中血凝素的生化特性

Biochemical properties of hemagglutinins in the mollusk Pomacea flagellata.

作者信息

Arreguín-Espinosa R, Arreguín-Lozano B

机构信息

Instituto de Química, Universidad Nacional Autónoma de México, Ciudad Universitaria, Coyoacán, México, D.F.

出版信息

Biochem Mol Biol Int. 1997 Dec;43(6):1241-51. doi: 10.1080/15216549700205071.

DOI:10.1080/15216549700205071

PMID:9442920

Abstract

Extracts of the Mollusk Pomacea flagellata were found to contain two agglutinins (PFA-I and PFA-II) which reacted with erythrocytes from human and several animal species. The hemagglutinating activity was stable between pH 4.0 and pH 10.0, and was independent of divalent cations. The active components were purified and found to be glycoproteins having Mr of 30,000. The isoelectric points were estimated to be 4.7 and 4.9 for PFA-I and PFA-II respectively. Hapten inhibition assays indicated that the agglutinin and its isoforms are specific for beta-galactosides as well as for galactose containing glycoproteins.

摘要

已发现福寿螺提取物含有两种凝集素（PFA - I和PFA - II），它们可与人及几种动物的红细胞发生反应。血凝活性在pH 4.0至pH 10.0之间稳定，且与二价阳离子无关。活性成分经纯化后发现是分子量为30,000的糖蛋白。PFA - I和PFA - II的等电点估计分别为4.7和4.9。半抗原抑制试验表明，该凝集素及其同工型对β - 半乳糖苷以及含半乳糖的糖蛋白具有特异性。