多头绒泡菌血凝素的纯化及某些性质

Purification and some properties of hemagglutinin from the Myxomycete, Physarum polycephalum.

作者信息

Yokota M, Nitta K

机构信息

Department of Health Sciences, Asahikawa College of Hokkaido University of Education, Japan.

出版信息

Experientia. 1996 Jun 15;52(6):544-8. doi: 10.1007/BF01969725.

DOI:10.1007/BF01969725

PMID:8698086

Abstract

A new hemagglutinin was isolated from the plasmodium of Physarum polycephalum by salting out with ammonium sulphate followed by chromatography on DE-32, DEAE-Toyopearl and hydroxyapatite. This hemagglutinin, named physarumin, was purified 1000-fold over crude extracts. The molecular weight of physarumin was determined to be 22,000 by size exclusion chromatography on Bio-Gel P-60 and 8,700 by SDS-polyacrylamide gel electrophoresis. Physarumin agglutinated rabbit, guinea pig, horse and human erythrocytes. Physarumin-induced hemagglutination was inhibited by fetuin and alpha 1-acid glycoprotein, but not by commercially available mono- and disaccharides. Hemagglutinating activity was blocked by EDTA, and was restored by adding Ca2+ but not by Mg2+.

摘要

通过硫酸铵盐析，随后在DE - 32、DEAE - Toyopearl和羟基磷灰石上进行色谱分离，从多头绒泡菌的疟原虫中分离出一种新的血凝素。这种血凝素被命名为绒泡菌血凝素，相对于粗提物纯化了1000倍。通过在Bio - Gel P - 60上进行尺寸排阻色谱法测定，绒泡菌血凝素的分子量为22,000，通过SDS - 聚丙烯酰胺凝胶电泳测定为8,700。绒泡菌血凝素能凝集兔、豚鼠、马和人的红细胞。胎球蛋白和α1 - 酸性糖蛋白可抑制绒泡菌血凝素诱导的血凝反应，但市售的单糖和双糖则不能。血凝活性被EDTA阻断，加入Ca2 +可恢复，但加入Mg2 +则不能。