Dubreuil P, Abribat T, Brazeau P, Lapierre H
Department of Clinical Sciences, College of Veterinary Medicine, St. Hyacinthe, Quebec.
Can J Vet Res. 1998 Jan;62(1):9-13.
A human growth hormone-releasing factor analog ([Desamino-Tyr1,D-Ala2,Ala15] hGRF(1-29) NH2) has been reported to reduce feed intake and increase growth and feed efficiency in a dose-dependent manner in growing pigs. The aim of this study was to determine the effect of this analog on nitrogen (N) balance and mineral excretion. Fifteen castrated male Yorkshire x Landrace pigs (45.9 +/- 1.4 kg) were randomly allotted to 2 groups: control (saline, n = 7) and GRF (6.66 micrograms/kg sc, TID, n = 8). The animals were injected for 20 consecutive days: feces and urine were collected during the last 10 d of injection. The animals had free access to water and food until satiety (approximately 15 min) at 07:00, 11:00, 15:00, 19:00, 23:00 and 07:00 h. The diet consisted of a hog fattening ration (18.0% crude protein). Blood samples were collected on the last day of the study by venipuncture. This analog increased (P < 0.05) insulin-like growth factor-1 and glucose serum concentrations and decreased (P < 0.05) serum urea nitrogen concentration and feed intake. The GRF-treated animals ingested less N, excreted less N in urine and feces to retain a similar amount of N than controls. The apparent coefficient of digestibility of the N has been slightly increased (P < 0.05) by GRF. Urinary excretion of P, K, and Cl decreased (P < 0.01) with GRF treatment. In conclusion, this GRF analog increased N digestibility and retention relative to N ingestion and reduced urinary N, P, K, and Cl excretion.
据报道，一种人生长激素释放因子类似物（[脱氨基酪氨酸1，D-丙氨酸2，丙氨酸15]hGRF(1-29)NH2）能以剂量依赖的方式降低生长猪的采食量，提高生长速度和饲料效率。本研究的目的是确定这种类似物对氮（N）平衡和矿物质排泄的影响。将15头去势的雄性约克夏×长白猪（45.9±1.4千克）随机分为2组：对照组（生理盐水，n = 7）和生长激素释放因子组（6.66微克/千克皮下注射，每日3次，n = 8）。连续20天给动物注射：在注射的最后10天收集粪便和尿液。动物在07:00、11:00、15:00、19:00、23:00和07:00可自由饮水和进食，直至饱腹（约15分钟）。日粮为育肥猪日粮（粗蛋白含量18.0%）。在研究的最后一天通过静脉穿刺采集血样。这种类似物提高了（P < 0.05）胰岛素样生长因子-1和血糖血清浓度，降低了（P < 0.05）血清尿素氮浓度和采食量。生长激素释放因子处理的动物摄入的氮较少，尿液和粪便中排泄的氮较少，与对照组相比保留了相似量的氮。生长激素释放因子使氮的表观消化系数略有提高（P < 0.05）。生长激素释放因子处理使磷、钾和氯的尿排泄量减少（P < 0.01）。总之，这种生长激素释放因子类似物相对于氮摄入增加了氮的消化率和保留率，并减少了尿中氮、磷、钾和氯的排泄。
