从大豆叶片中鉴定出两个胞质抗坏血酸过氧化物酶cDNA，并通过在大肠杆菌中的功能表达对其产物进行表征。
Identification of two cytosolic ascorbate peroxidase cDNAs from soybean leaves and characterization of their products by functional expression in E. coli.
作者信息
Caldwell C R, Turano F J, McMahon M B
机构信息
Climate Stress Laboratory, Beltsville Agricultural Research Center, United States Department of Agriculture, MD 20705-2350, USA.
出版信息
Planta. 1998 Jan;204(1):120-6. doi: 10.1007/s004250050237.
Screening of a cDNA library from soybean (Glycine max (L.) Merr. cv. Century) with probes based upon cytosolic ascorbate peroxidase (APx; EC 1.11.1.11) genes identified two full-length clones (SOYAPx1, SOYAPx2) apparently encoding for different soybean leaf cytosolic APxs. The deduced amino acid sequences of the two APx cDNA products differed in 13 of the 250 amino acids. The SOYAPx1 cDNA was identical to the cytosolic APx cDNA previously found in soybean root nodules. Escherichia coli expression systems were developed using both soybean APx cDNAs. Recombinant SOYAPx1 and SOYAPx2 were then utilized to characterize the enzymatic properties of the two APx cDNA products.
用基于胞质抗坏血酸过氧化物酶（APx；EC 1.11.1.11）基因的探针筛选大豆（Glycine max (L.) Merr. cv. Century）的cDNA文库，鉴定出两个全长克隆（SOYAPx1、SOYAPx2），它们显然编码不同的大豆叶片胞质APx。两种APx cDNA产物推导的氨基酸序列在250个氨基酸中有13个不同。SOYAPx1 cDNA与先前在大豆根瘤中发现的胞质APx cDNA相同。利用这两种大豆APx cDNA构建了大肠杆菌表达系统。然后用重组SOYAPx1和SOYAPx2来表征这两种APx cDNA产物的酶学性质。
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