Siegan J B, Sagen J
Department of Anatomy and Cell Biology, University of Illinois at Chicago, 60612, USA.
Pharmacol Biochem Behav. 1998 Jan;59(1):97-104. doi: 10.1016/s0091-3057(97)00326-2.
Previous work in our laboratory has demonstrated that adrenal medullary transplants into the spinal subarachnoid space can alleviate neuropathic pain behaviors. The purpose of this study was to test the possibility that motor, as well as, sensory dysfunction is reduced by adrenal medullary transplants. Peripheral neuropathy was induced by a chronic constriction injury (CCI) of the sciatic nerve of rats. In addition to exaggerated responses to noxious and innocuous stimuli characteristic of peripheral nerve injury, severe impairment of hindpaw placing and grasping reflexes following CCI was observed. Two weeks following CCI, either adrenal medullary or control striated muscle tissue was implanted into the spinal subarachnoid space. Adrenal medullary, but not control transplants, produced significant restoration of hindlimb reflex function in animals with peripheral nerve injury. This was reversed by pretreatment with the alpha-adrenergic antagonist phentolamine, but not the opiate antagonist naloxone, suggesting a role for catecholamines secreted by the implanted cells in reflex recovery. Adrenal medullary transplants also attenuated hyperalgesia and allodynia resulting from nerve injury. These results indicate that adrenal medullary transplants can alleviate sensorimotor dysfunction consequent to peripheral nerve injury.
我们实验室之前的研究表明，将肾上腺髓质移植到脊髓蛛网膜下腔可缓解神经性疼痛行为。本研究的目的是测试肾上腺髓质移植是否能减轻运动功能障碍以及感觉功能障碍。通过对大鼠坐骨神经进行慢性压迫损伤（CCI）来诱导周围神经病变。除了对周围神经损伤特有的有害和无害刺激反应过度外，还观察到CCI后后爪放置和抓握反射严重受损。CCI两周后，将肾上腺髓质或对照横纹肌组织植入脊髓蛛网膜下腔。肾上腺髓质移植而非对照移植显著恢复了周围神经损伤动物的后肢反射功能。用α-肾上腺素能拮抗剂酚妥拉明预处理可逆转这种情况，但阿片拮抗剂纳洛酮则不能，这表明植入细胞分泌的儿茶酚胺在反射恢复中起作用。肾上腺髓质移植还减轻了神经损伤引起的痛觉过敏和异常性疼痛。这些结果表明，肾上腺髓质移植可缓解周围神经损伤导致的感觉运动功能障碍。
