Higuchi S, Tabata N, Tajima M, Ito M, Tsurudome M, Sudo A, Uchida A, Ito Y
Department of Microbiology, Mie University School of Medicine, Japan.
J Bone Miner Res. 1998 Jan;13(1):44-9. doi: 10.1359/jbmr.1998.13.1.44.
We have developed a new and simple system of human osteoclast formation by fusing peripheral blood monocytes with anti-Fusion Regulatory Protein-1 (anti-FRP-1) monoclonal antibody (mAb). When human blood monocytes were cultured in the presence of anti-FRP-1/CD98 mAbs, polykaryocytes began to appear at approximately 15 h and increased in size with time until 3-4 days of incubation with anti-FRP-1 mAb. These fused cells showed positive staining in tartrate-resistant acid phosphatase, possessed numerous calcitonin receptors, and were capable of bone resorption. These results strongly suggest that anti-FRP-1 antibody-induced multinucleated cells are osteoclasts. Furthermore, FRP-1 antigens were detected in osteoclasts isolated from human bone and in the osteoclast-like cells obtained from human giant cell tumors of bone.
我们通过将外周血单核细胞与抗融合调节蛋白-1（anti-FRP-1）单克隆抗体（mAb）融合，开发了一种新的、简单的人破骨细胞形成系统。当人血单核细胞在抗FRP-1/CD98 mAb存在下培养时，多核细胞在大约15小时开始出现，并随着时间推移而增大，直至与抗FRP-1 mAb孵育3 - 4天。这些融合细胞在抗酒石酸酸性磷酸酶染色中呈阳性，拥有大量降钙素受体，并且能够进行骨吸收。这些结果有力地表明，抗FRP-1抗体诱导的多核细胞是破骨细胞。此外，在从人骨分离的破骨细胞以及从人骨巨细胞瘤获得的破骨细胞样细胞中检测到了FRP-1抗原。
