Chuansumrit A, Siripoonya P, Nathalang O, Sriphaisal T
Department of Pediatrics, Faculty of Medicine Ramathibodi Hospital, Bangkok, Thailand.
Southeast Asian J Trop Med Public Health. 1997 Jun;28(2):428-31.
The direct antiglobulin test (DAT) using the gel technique was included in the investigation of infants with hyperbilirubinemia in the first week of life. Twelve cases were preterm and 48 cases were full term infants. The patients were divided into 2 groups: the study group comprised 22 cases of blood group A or B infants born to blood group O mothers; the control group comprised 38 cases of ABO blood groups compatible with those of their mothers. The mean +/- SD (39 +/- 26 hours) of the age at the onset of hyperbilirubinemia induced by ABO hemolytic disease of the newborn (HDN) was significantly earlier than that due to other causes. The positive rate of DAT in the ABO incompatible group was similar by both the conventional technique and the gel technique, 54.5% and 50% respectively. However, the scores by the gel technique were higher than those of the conventional technique. The gel technique is simple, reliable, involves less technical error and requires a small amount of blood sample. The grading system is clear-cut, especially grade 1 + or weak positive as compared to the conventional technique which requires examination under a microscope. Therefore, the DAT using the gel technique is beneficial to the diagnosis of ABO HDN. It should be included in the investigation of infants with hyperbilirubinemia especially in case of suspected ABO HDN.
采用凝胶技术的直接抗球蛋白试验（DAT）被纳入对出生后第一周内患有高胆红素血症婴儿的调查中。12例为早产儿，48例为足月儿。患者被分为两组：研究组包括22例母亲为O型血而出生的A型或B型血婴儿；对照组包括38例ABO血型与其母亲血型相符的婴儿。新生儿ABO溶血病（HDN）所致高胆红素血症发病时的平均年龄±标准差（39±26小时）显著早于其他原因所致者。ABO血型不合组中，传统技术和凝胶技术检测DAT的阳性率相似，分别为54.5%和50%。然而，凝胶技术的评分高于传统技术。凝胶技术简单、可靠，技术误差小，所需血样量少。分级系统明确，尤其是1 +级或弱阳性，与需要在显微镜下检查的传统技术相比更为明显。因此，采用凝胶技术的DAT有助于ABO HDN的诊断。它应被纳入对患有高胆红素血症婴儿的调查中，尤其是在怀疑ABO HDN的情况下。
