Rychlewski T, Szcześniak L, Dylewicz P, Deskur E, Przywarska I, Kasprzak Z, Karolkiewicz J, Konys L
Chair of Physiology, Biochemistry and Hygiene, University School of Physical Education, Poznań, Poland.
J Physiol Pharmacol. 1997 Dec;48(4):839-49.
In this study, we investigated the influence of glucose administration on binding and degradation of 125I-insulin by receptors on erythrocytes as well as on insulin and C-peptide serum levels in 15 patients after myocardial infarction and in 15 age-matched healthy persons. Venous blood samples were taken directly before and at 30, 60 and 120 minutes after oral administration of 75 g of glucose. In the collected blood samples serum glucose, insulin and C-peptide levels were determined. Binding and degradation of 125I-insulin by specific receptors on red blood cells were evaluated using the method described by Gambhir and modified by the authors. Serum insulin and C-peptide levels were significantly higher while binding of 125I-insulin to erythrocytes was decreased in patients after myocardial infarction. These results seem to support the hypothesis that insulin resistance and hyperinsulinism play a role in the pathogenesis of ischaemic heart disease. Impaired degradation of 125I-insulin during the oral glucose tolerance test in the patients after myocardial infarction indicates that insulin resistance is located at the receptor level.
在本研究中，我们调查了给予葡萄糖对15例心肌梗死后患者及15例年龄匹配的健康人的红细胞受体结合及降解125I胰岛素的影响，以及对胰岛素和C肽血清水平的影响。在口服75克葡萄糖之前及之后30、60和120分钟直接采集静脉血样本。测定所采集血样中的血清葡萄糖、胰岛素和C肽水平。使用Gambhir所描述并经作者修改的方法评估红细胞上特异性受体对125I胰岛素的结合及降解情况。心肌梗死后患者血清胰岛素和C肽水平显著升高，而125I胰岛素与红细胞的结合减少。这些结果似乎支持了胰岛素抵抗和高胰岛素血症在缺血性心脏病发病机制中起作用这一假说。心肌梗死后患者口服葡萄糖耐量试验期间125I胰岛素降解受损表明胰岛素抵抗位于受体水平。
