Tamura A, Hebisawa A, Komatsu H, Yotsumoto H, Mori M
Department of Respiratory Diseases, Tokyo National Chest Hospital, Japan.
Lung Cancer. 1997 Nov;18(3):253-7. doi: 10.1016/s0169-5002(97)00066-4.
To investigate whether p53 and proliferating cell nuclear antigen (PCNA) are maintained at relapse of non-small cell lung cancer (NSCLC), we examined tumor materials from nine patients with NSCLC who had undergone resection for primary cancer and also a second resection for its relapse to the lung. In each case, histological types of primary and relapsing tumor were identical (eight adenocarcinomas and one squamous cell carcinoma). Immunohistochemical staining analysis for p53 oncoprotein expression revealed that seven of the nine cases had identical p53 expression in primary and relapsing tumor (p53 positive in three cases and negative in four) and that in the remaining two cases, p53 positive conversion during relapse was found in one case and negative conversion in one. Immunostaining for PCNA expression revealed that PCNA expression was observed in five primary tumors, and at relapse these cases were also PCNA positive. Three of the remaining four cases showed PCNA positive conversion during relapse. This study of a small number of patients indicates that results of p53 and PCNA immunostaining of resected materials of NSCLC seem to be of little significance for predicting future relapse.
为了研究非小细胞肺癌（NSCLC）复发时p53和增殖细胞核抗原（PCNA）的状态，我们检测了9例NSCLC患者的肿瘤组织。这些患者均接受了原发性癌症切除手术，之后又因肿瘤复发至肺部而接受了二次切除手术。在每例患者中，原发性肿瘤和复发性肿瘤的组织学类型均相同（8例腺癌和1例鳞状细胞癌）。对p53癌蛋白表达进行的免疫组织化学染色分析显示，9例病例中有7例原发性肿瘤和复发性肿瘤的p53表达相同（3例p53阳性，4例阴性），其余2例中，1例在复发时出现p53阳性转化，1例出现阴性转化。对PCNA表达进行的免疫染色显示，5例原发性肿瘤中观察到PCNA表达，复发时这些病例也为PCNA阳性。其余4例中的3例在复发时出现PCNA阳性转化。这项对少数患者的研究表明，NSCLC切除组织的p53和PCNA免疫染色结果对于预测未来复发似乎意义不大。
