Baz A, Henry L, Chateau M T, Scherrer K, Bureau J P
Laboratoire de Biologie Cellulaire et Cytogénétique Moléculaire (UPRES-JE 1952), Faculté de Médecine Montpellier-Nimes, Nimes, France.
Leuk Res. 1997 Nov-Dec;21(11-12):1061-70. doi: 10.1016/s0145-2126(97)00091-x.
The human lymphoblastoid leukemic cell line (CCRF-CEM) was induced to differentiate with phorbol 12-myristate 13-acetate (PMA). During differentiation, assessed by monitoring the cluster of differentiation (CD) profile, the prosome (proteasomes, multi-catalytic proteinase) distribution and composition were studied by microscopy, flow cytometry and Western blot analysis. Changes in prosome subunits were monitored using 3 monoclonal antibodies anti-p23K, p29K and p31K. There were changes in the subcellular distribution of prosome antigens in PMA treated cells compared to untreated cells. The amount of cytoplasmic prosomal antigens decreased during the first three days of differentiation and the membrane antigens increased; meanwhile there was an increase of p53 and no change in actin protein levels. As mitotic cyclins are degraded by the ubiquitin pathway and therefore via the prosome, the decrease observed in differentiated cells suggests that prosomes are involved in the cell cycle and thus in cell proliferation.
用人淋巴细胞白血病细胞系（CCRF-CEM），通过佛波酯12-肉豆蔻酸酯13-乙酸酯（PMA）诱导其分化。在分化过程中，通过监测分化簇（CD）谱进行评估，运用显微镜、流式细胞术和蛋白质免疫印迹分析，研究蛋白酶体（蛋白酶体，多催化蛋白酶）的分布和组成。使用3种抗p23K、p29K和p31K的单克隆抗体监测蛋白酶体亚基的变化。与未处理的细胞相比，PMA处理的细胞中蛋白酶体抗原的亚细胞分布发生了变化。在分化的前三天，细胞质蛋白酶体抗原的量减少，膜抗原增加；同时，p53增加，肌动蛋白蛋白水平没有变化。由于有丝分裂周期蛋白通过泛素途径进而通过蛋白酶体降解，在分化细胞中观察到的减少表明蛋白酶体参与细胞周期，从而参与细胞增殖。
