Roller R J, Rauch D
Department of Microbiology, University of Iowa, Iowa City 52242, USA.
J Virol. 1998 Feb;72(2):1411-7. doi: 10.1128/JVI.72.2.1411-1417.1998.
95-19 and U(S)11c119.3 are BHK(TK-)-derived cell lines that are highly resistant to postattachment entry of herpes simplex virus type 1 (HSV-1) and HSV-2 but not to later steps in single-step replication. The resistance properties of these two cell types are not identical. U(S)11c119.3 cells are fully susceptible to pseudorabies virus (PRV), as shown by single-step growth experiments, whereas 95-19 cells are resistant to entry of free PRV but not to entry by cell-cell spread. We have tested the ability of HVEM to overcome the block to infection in both cell lines following transient and stable transfection. HVEM was able to mediate entry of free HSV-1 into both cell lines, as shown by an increase in the number of beta-galactosidase-expressing cells in cultures transiently transfected with an HVEM expression plasmid and infected with lacZ-expressing HSV-1. In stably transfected 95-19 cells, HVEM enhanced infection by free HSV-1, as shown by an increase in the number of infectious centers obtained following infection. In both cell types, HVEM strongly enhanced entry of HSV-1 and HSV-2 by cell-cell spread, suggesting that HVEM can function as an entry mediator both in entry of free virus and in entry by cell-cell spread.
95 - 19和U(S)11c119.3是源自BHK(TK -)的细胞系，它们对单纯疱疹病毒1型（HSV - 1）和HSV - 2的附着后进入具有高度抗性，但对单步复制的后续步骤不具有抗性。这两种细胞类型的抗性特性并不相同。如单步生长实验所示，U(S)11c119.3细胞对伪狂犬病病毒（PRV）完全敏感，而95 - 19细胞对游离PRV的进入具有抗性，但对细胞 - 细胞传播的进入不具有抗性。我们测试了在瞬时转染和稳定转染后，HVEM克服这两种细胞系感染障碍的能力。如用HVEM表达质粒瞬时转染并用表达lacZ的HSV - 1感染的培养物中表达β - 半乳糖苷酶的细胞数量增加所示，HVEM能够介导游离HSV - 1进入这两种细胞系。在稳定转染的95 - 19细胞中，如感染后获得的感染中心数量增加所示，HVEM增强了游离HSV - 1的感染。在这两种细胞类型中，HVEM都强烈增强了HSV - 1和HSV - 2通过细胞 - 细胞传播的进入，这表明HVEM在游离病毒进入和细胞 - 细胞传播进入过程中都可以作为进入介质发挥作用。
