Garcea N, Caruso A, Campo S, Milano L, Bompiani A
Fertil Steril. 1976 Apr;27(4):442-8. doi: 10.1016/s0015-0282(16)41783-8.
The authors have incubated, in vitro, estrous rabbit endometrium with 3H-leucine and pseudopregnant rabbit endometrium with 14C-leucine. By the double-labeling technique and the use of the 14C:3H ratio, it was shown that blastokinin (BKN) is neosynthesized in vitro and that this neosynthesis probably also affects other protein components. By ion exchange chromatography of the supernatant of the pseudopregnant endometrium and previously purified BKN, it was possible to confirm the in vitro biosynthesis and to exclude interfering phenomena. Time dynamics showed that biosynthesis was more marked between the 18th and 24th hours of incubation.
作者们分别用³H-亮氨酸体外孵育发情期兔子宫内膜，用¹⁴C-亮氨酸体外孵育假孕兔子宫内膜。通过双标记技术以及¹⁴C:³H比值的运用，结果表明，胚激肽（BKN）在体外重新合成，且这种重新合成可能也影响其他蛋白质成分。通过对假孕子宫内膜上清液和先前纯化的BKN进行离子交换色谱分析，得以证实体外生物合成并排除干扰现象。时间动态分析表明，在孵育的第18至24小时之间生物合成更为显著。
