细胞周期蛋白E过表达通过p21WAF1/CIP1/SDI1促进人类肝脏肿瘤生长。
Cyclin E overexpression responsible for growth of human hepatic tumors with p21WAF1/CIP1/SDI1.
作者信息
Tsuji T, Miyazaki M, Fushimi K, Mihara K, Inoue Y, Ohashi R, Ohtsubo M, Hamazaki K, Furusako S, Namba M
机构信息
Department of Cell Biology, Institute of Molecular and Cellular Biology, Okayama University Medical School.
出版信息
Biochem Biophys Res Commun. 1998 Jan 14;242(2):317-21. doi: 10.1006/bbrc.1997.7958.
We examined a relationship between p21WAF1/CIP1/SDI1 and cell-cycle-related proteins in 12 human liver tumor cell lines (JHH-1, -2, -4, -5, -6, -7; HLE; HuH-7; Hep3B; PLC/PRF/5; HuH-6; HepG2). Seven (JHH-1, -2, -5, -6, -7; Hep3B; HepG2) out of eight cell lines having p21WAF1/CIP1/SDI1 protein overexpressed cyclin E protein, although one of them (JHH-5) overexpressed a reduced size of cyclin E. The rest (HuH-6) of the 8 cell lines with p21WAF1/CIP1/SDI1 showed a decreased expression of cyclin E. Four cell lines (JHH-4; HLE; HuH-7; PLC/PRF/5) deficient of p21WAF1/CIP1/SDI1 protein did not overexpress cyclin E protein. As to expression of the other cell-cycle-related proteins, cyclin A, cyclin D1, CDK2 or CDK4, no significant difference was detected among the 12 cell lines. These findings indicate that the human liver tumor cell lines which have the p21WAF1/CIP1/SDI1-inducible barriers of the cell cycle progression can go through the G1/S checkpoint by overexpressing cyclin E.
我们检测了12种人肝癌细胞系（JHH-1、-2、-4、-5、-6、-7；HLE；HuH-7；Hep3B；PLC/PRF/5；HuH-6；HepG2）中p21WAF1/CIP1/SDI1与细胞周期相关蛋白之间的关系。在8种p21WAF1/CIP1/SDI1蛋白过表达的细胞系中，有7种（JHH-1、-2、-5、-6、-7；Hep3B；HepG2）细胞周期蛋白E蛋白过表达，尽管其中一种（JHH-5）过表达的细胞周期蛋白E大小有所减小。8种p21WAF1/CIP1/SDI1阳性细胞系中的其余细胞系（HuH-6）细胞周期蛋白E表达降低。4种缺乏p21WAF1/CIP1/SDI1蛋白的细胞系（JHH-4；HLE；HuH-7；PLC/PRF/5）未过表达细胞周期蛋白E蛋白。至于其他细胞周期相关蛋白，即细胞周期蛋白A、细胞周期蛋白D1、CDK2或CDK4的表达，在这12种细胞系中未检测到显著差异。这些发现表明，具有p21WAF1/CIP1/SDI1诱导的细胞周期进展障碍的人肝癌细胞系可以通过过表达细胞周期蛋白E来通过G1/S关卡。
Zotero 插件