在核黄素存在的情况下，高效液相色谱法测定叶酸及其光降解产物。
High performance liquid chromatographic determination of folic acid and its photodegradation products in the presence of riboflavin.
作者信息
Akhtar M J, Khan M A, Ahmad I
机构信息
Department of Chemistry, University of Karachi, Pakistan.
出版信息
J Pharm Biomed Anal. 1997 Sep;16(1):95-9. doi: 10.1016/s0731-7085(96)02028-6.
A high performance liquid chromatographic procedure was developed to determine folic acid and its photodegradation products, p-aminobenzoic acid, pterine-6-carboxylic acid, p-aminobenzoyl-L-glutamic acid, and pteroic acid in the presence of riboflavin. The method involves reversed phase, paired-ion chromatography on mu-BondaPak C18 column using a UV detector (254 nm), and isocratic solvent system (at ambient temperature) comprising 0.017 M monobasic potassium phosphate, tetrabutyl ammonium hydroxide solution (20%, aqueous) and methanol (870:15:250, v/v). The range of quantitation for the individual compounds was found to be: p-aminobenzoic acid, 0.01 - 1.25 x 10(-5) M; pterine-6-carboxylic acid, 0.01-2.0 x 10(-5) M; p-aminobenzoyl-L-glutamic acid, 0.02-2.0 x 10(-5) M; pteroic acid, 0.02-2.5 x 10(-5) M; folic acid, 1.0-5.0 x 10(-5) M; riboflavin, 1.0-5.0 x 10(-5) M. Linear regression analysis of the data demonstrates adequate performance of the method in terms of accuracy and precision (R. S. D. 3%). The method is specific, rapid and convenient and has been applied to photodegradation studies of folic acid in the presence and absence of riboflavin.
建立了一种高效液相色谱法，用于在核黄素存在的情况下测定叶酸及其光降解产物对氨基苯甲酸、蝶呤 - 6 - 羧酸、对氨基苯甲酰 - L - 谷氨酸和蝶酸。该方法采用反相、离子对色谱法，在μ - BondaPak C18柱上使用紫外检测器（254 nm），等度溶剂系统（常温）由0.017 M磷酸二氢钾、氢氧化四丁铵溶液（20%，水溶液）和甲醇（870:15:250，v/v）组成。发现各化合物的定量范围为：对氨基苯甲酸，0.01 - 1.25×10⁻⁵ M；蝶呤 - 6 - 羧酸，0.01 - 2.0×10⁻⁵ M；对氨基苯甲酰 - L - 谷氨酸，0.02 - 2.0×10⁻⁵ M；蝶酸，0.02 - 2.5×10⁻⁵ M；叶酸，1.0 - 5.0×10⁻⁵ M；核黄素，1.0 - 5.0×10⁻⁵ M。数据的线性回归分析表明该方法在准确性和精密度方面表现良好（相对标准偏差为3%）。该方法具有特异性、快速且方便，已应用于在有和没有核黄素存在的情况下叶酸的光降解研究。
Zotero 插件