高效液相色谱法同时分析血浆中利多卡因和布比卡因对映体
Simultaneous analysis of lignocaine and bupivacaine enantiomers in plasma by high-performance liquid chromatography.
作者信息
Abraham I, Fawcett J P, Kennedy J, Kumar A, Ledger R
机构信息
School of Pharmacy, University of Otago, Dunedin, New Zealand.
出版信息
J Chromatogr B Biomed Sci Appl. 1997 Dec 5;703(1-2):203-8. doi: 10.1016/s0378-4347(97)00420-9.
A sensitive analytical procedure is described for the simultaneous determination of lignocaine and the enantiomers of bupivacaine in biological fluids using diazepam as an internal standard. After solvent extraction into hexane, the local anaesthetics were separated using an alpha1-acid glycoprotein (AGP) column and detected at 214 nm. Calibration curves were linear (r2>0.99) in the concentration range of 5 to 500 ng/ml for the enantiomers of bupivacaine and 12.5 to 1000 ng/ml for lignocaine. The corresponding limits of detection were 4 ng/ml and 10 ng/ml, respectively. The method was applied to the analysis of plasma from a healthy woman undergoing tubal ligation.
描述了一种灵敏的分析方法，以地西泮为内标，同时测定生物流体中的利多卡因和布比卡因对映体。用己烷进行溶剂萃取后，使用α1-酸性糖蛋白（AGP）柱分离局部麻醉剂，并在214nm处进行检测。布比卡因对映体浓度范围为5至500ng/ml，利多卡因浓度范围为12.5至1000ng/ml时，校准曲线呈线性（r2>0.99）。相应的检测限分别为4ng/ml和10ng/ml。该方法应用于对一名接受输卵管结扎的健康女性的血浆分析。
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