Bugajska-Schretter A, Elfman L, Fuchs T, Kapiotis S, Rumpold H, Valenta R, Spitzauer S
Institute of Medical and Chemical Laboratory Diagnostics, AKH, University of Vienna, Austria.
J Allergy Clin Immunol. 1998 Jan;101(1 Pt 1):67-74. doi: 10.1016/S0091-6749(98)70195-2.
Type I allergy to fish is a severe health problem in countries in which a large percentage of the population derive income from fishing.
The aim of the study was to characterize cross-reactive IgE-binding components in six different fish species (cod, tuna, salmon, perch, carp, and eel). The effect of reducing extraction conditions, periodate treatment, and depletion of Ca2+ on binding of IgE to the allergens was investigated.
Extracts were prepared under nonreducing and reducing conditions. IgE-binding components were characterized by IgE immunoblotting, and cross-reactive epitopes were studied by IgE-immunoblot inhibition experiments. To reveal calcium-sensitive or carbohydrate-containing epitopes, nitrocellulose-blotted extracts were exposed to ethylene glycol bis(beta-aminoethyl ether)-N,N,N',N'-tetraacetic acid (EGTA) and periodate.
Sera from all patients allergic to fish (n = 30) displayed IgE reactivity to parvalbumin, a 12 kd protein present in fish extracts from six different species. Reducing extraction conditions had no effect on IgE binding to parvalbumins, whereas periodate treatment and depletion of protein-bound calcium led to a substantial reduction of IgE binding. Parvalbumins from six different species contained cross-reactive IgE epitopes.
Parvalbumin represents a cross-reactive fish allergen. It contains IgE epitopes that are sensitive to periodate treatment and Ca2+-depletion.
在很大一部分人口以捕鱼为生的国家，鱼类I型过敏是一个严重的健康问题。
本研究旨在鉴定六种不同鱼类（鳕鱼、金枪鱼、鲑鱼、鲈鱼、鲤鱼和鳗鱼）中交叉反应性IgE结合成分。研究了降低提取条件、高碘酸盐处理和去除Ca2+对IgE与过敏原结合的影响。
在非还原和还原条件下制备提取物。通过IgE免疫印迹法鉴定IgE结合成分，并通过IgE免疫印迹抑制实验研究交叉反应表位。为了揭示钙敏感或含碳水化合物的表位，将硝酸纤维素膜印迹提取物暴露于乙二醇双（β-氨基乙醚）-N，N，N'，N'-四乙酸（EGTA）和高碘酸盐中。
所有对鱼类过敏的患者（n = 30）的血清均显示出对小清蛋白的IgE反应性，小清蛋白是一种存在于六种不同鱼类提取物中的12 kd蛋白。降低提取条件对IgE与小清蛋白的结合没有影响，而高碘酸盐处理和去除蛋白结合的钙导致IgE结合显著减少。六种不同鱼类的小清蛋白含有交叉反应性IgE表位。
小清蛋白是一种交叉反应性鱼类过敏原。它含有对高碘酸盐处理和Ca2+去除敏感的IgE表位。
