Pauwels W, Farmer P B, Osterman-Golkar S, Severi M, Cordero R, Bailey E, Veulemans H
Laboratory for Occupational Hygiene and Toxicology, Katholieke Universiteit Leuven, Belgium.
J Chromatogr B Biomed Sci Appl. 1997 Nov 21;702(1-2):77-83. doi: 10.1016/s0378-4347(97)00380-0.
A ring-test was organised between three laboratories using different versions of the modified Edman degradation technique for the gas chromatographic-mass spectrometric determination of N-terminal valine adducts of styrene 7,8-oxide. The analyses were performed on a sample of human haemoglobin reacted in vitro with styrene 7,8-oxide and on a set of five haemoglobin samples from mice dosed by i.p. injection of styrene. Strong correlations between the haemoglobin adduct determinations of the different laboratories were observed. However, covariance analysis revealed different slopes for the dose-response curves, indicating differences for the calibration of the reference globin or reference peptide.
在三个实验室之间组织了一次环形测试，使用不同版本的改良埃德曼降解技术，通过气相色谱 - 质谱法测定苯乙烯7,8 - 氧化物的N - 末端缬氨酸加合物。分析针对的是在体外与苯乙烯7,8 - 氧化物反应的人血红蛋白样品，以及通过腹腔注射苯乙烯给药的一组五只小鼠的血红蛋白样品。观察到不同实验室之间血红蛋白加合物测定结果具有很强的相关性。然而，协方差分析显示剂量 - 反应曲线的斜率不同，表明参考珠蛋白或参考肽的校准存在差异。
