Vendramin G G, Ziegenhagen B
Instituto Miglioramento Genetico delle Piante Forestali, Consiglio Nazionale delle Ricerche, Florence, Italy.
Genome. 1997 Dec;40(6):857-64. doi: 10.1139/g97-811.
Two polymorphic microsatellite loci were identified and sequenced in the genus Abies, using primer pairs derived from chloroplast simple sequence repeats (SSRs) of Pinus thunbergii. PCR products exhibited considerable length variation among six different Abies species and within Abies alba. F1 progeny of both an interspecific and an intraspecific reciprocal cross confirmed that the two SSRs were predominantly paternally inherited. The maternal size variant predominantly occurred in the megagametophytes analysed. First analysis of the two chloroplast microsatellites in seven natural populations of A. alba revealed 36 different haplotypes. The use of these highly polymorphic SSRs as potential markers in population genetics is discussed.
利用源自黑松叶绿体简单序列重复（SSR）的引物对，在冷杉属中鉴定并测序了两个多态性微卫星位点。PCR产物在六种不同的冷杉物种之间以及欧洲冷杉内部表现出相当大的长度变异。种间和种内正反交的F1后代证实，这两个SSR主要是父系遗传的。母本大小变异主要出现在所分析的雌配子体中。对欧洲冷杉七个自然种群中的两个叶绿体微卫星的首次分析揭示了36种不同的单倍型。讨论了将这些高度多态性的SSR用作种群遗传学潜在标记的用途。
