Yoshida M, Nakagaki K, Nogami S, Harasawa R, Maeda R, Katae H, Hayashi Y
Animal Science Division, Dainippon Pharmaceutical Co., Ltd., Osaka, Japan.
J Vet Med Sci. 1997 Dec;59(12):1115-21. doi: 10.1292/jvms.59.1115.
This study was conducted to evaluate protective efficiency of three different protocols for vaccination in canine heartworm infection. To evaluate the three protocols of immunization, dogs were separately immunized with living larvae; 1) immunization with gamma-attenuated infective larvae, 2) with 50 micrograms/kg ivermectin-abbreviation, and 3) with chemical abbreviation plus Freund's complete adjuvant (FCA). Each group was composed of two dogs. All dogs used for this study were subcutaneously challenged with 100 intact third-stage larvae (L3) various days after the last immunization, and the worms in the pulmonary arteries and the right ventricle of the heart were recovered 17 to 25 weeks post-infection. The numbers and the sexes of the worms were determined. A mean of 38 worms was burdened in the group immunized with irradiated L3, 36 worms in the chemically-abbreviated group, but 15.5 worms in the group with chemical abbreviation plus FCA. The percentages of the protection in the former two groups were nearly 50%, but 72.3% in the group with ivermectin plus FCA. The adjuvant enhanced the protective immunity against L3 challenge. Obvious eosinophilia was observed in both immunized and control dogs except for two dogs. There was no correlation between the suppression of eosinophilia and the protective immunity in the present study.
本研究旨在评估三种不同疫苗接种方案对犬心丝虫感染的保护效果。为评估这三种免疫方案，将犬分别用活幼虫进行免疫；1）用γ-减毒感染性幼虫免疫，2）用50微克/千克伊维菌素（缩写）免疫，3）用化学缩写物加弗氏完全佐剂（FCA）免疫。每组由两只犬组成。用于本研究的所有犬在最后一次免疫后的不同天数皮下接种100条完整的第三期幼虫（L3），并在感染后17至25周回收肺动脉和右心室中的虫体。确定虫体的数量和性别。用辐照L3免疫的组平均负担38条虫，化学缩写组为36条虫，而化学缩写加FCA组为15.5条虫。前两组的保护百分比接近50%，但伊维菌素加FCA组为72.3%。佐剂增强了针对L3攻击的保护性免疫。除两只犬外，在免疫犬和对照犬中均观察到明显的嗜酸性粒细胞增多。在本研究中，嗜酸性粒细胞增多的抑制与保护性免疫之间没有相关性。
