Murakami M, Fujitani H
Laboratory of Molecular Biology, Azabu University School of Veterinary Medicine, Kanagawa, Japan.
Zoolog Sci. 1997 Oct;14(5):763-9. doi: 10.2108/zsj.14.763.
A family of highly repetitive DNAs (Hi-a) in the genome from a population of the crucian carp, tentatively identified as the ginbuna (Carassius auratus langsdorfi), was isolated from the HindIII digests and characterized. The Hi-a monomer (268 or 269 bp) was AT-rich (64.9%) with internal repetitive oligomers. The nucleotide similarity among monomers within the same individual was 88-98%, whose sequence alterations occurred mostly at restricted sites. Hybridization analyses revealed that the Hi-a family was organized into tandem array(s) representing satellite DNAs, and that its variants that share certain restriction sites of the repeat unit were dispersed into the tandem array(s) of Hi-a DNAs at varying periodicities. The lack of sequences homologous to the ginbuna Hi-a in the genomes from cyprinid fishes other than gengorobuna and goldfish suggests that Hi-a DNAs are specifically present in the species C. auratus. The genome of goldfish differed somewhat in the quantity or the genomic organization of Hi-a DNAs from that of the ginbuna.
从鲫鱼（暂定名为银鲫，即Carassius auratus langsdorfi）群体的基因组中分离并鉴定了一个高度重复DNA家族（Hi-a），该家族来自HindIII酶切片段。Hi-a单体（268或269 bp）富含AT（64.9%），含有内部重复寡聚物。同一个体内单体之间的核苷酸相似性为88 - 98%，其序列改变大多发生在受限位点。杂交分析表明，Hi-a家族被组织成代表卫星DNA的串联阵列，并且其共享重复单元某些限制性位点的变体以不同周期分散到Hi-a DNA的串联阵列中。除了日本丁鱥和金鱼外，鲤科鱼类基因组中缺乏与银鲫Hi-a同源的序列，这表明Hi-a DNA特异性存在于银鲫物种中。金鱼的基因组在Hi-a DNA的数量或基因组组织方面与银鲫有所不同。
