Kohnert K D, Wohlrab F, Hahn H J, Cossel L
Institute of Pathophysiology, University of Greifswald, Karlsburg, Germany.
Acta Diabetol. 1997 Dec;34(4):301-4. doi: 10.1007/s005920050092.
Syngeneic islets were transplanted into the liver of streptozotocin (STZ)-induced diabetic LEW.1W rats, and the expression of the glucose transporter isoform GLUT 2, an essential component of the glucose-sensing mechanism of the pancreatic beta-cell, was determined in the grafted islet tissue. Graft-bearing liver was obtained 12, 36, and 60 weeks after transplantation, and tissue sections were immunoperoxidase stained for GLUT 2 and major islet peptides. Islet cell aggregates of different sizes were found in the portal tract and in juxtaposition to the hepatocytes. At all time points, beta-cells in the grafts displayed GLUT 2 expression comparable to that of islets in nondiabetic rats. Islet cells containing immunoreactive insulin and islet amyloid polypeptide were plentiful, while those staining positive for glucagon and somatostatin were scarce in these grafts. The results show that beta-cells in islets engrafted in the liver, although initially exposed to chronic hyperglycemia, have the capability of stably expressing GLUT 2 over long-term periods.
将同基因胰岛移植到链脲佐菌素（STZ）诱导的糖尿病LEW.1W大鼠的肝脏中，并在移植的胰岛组织中测定葡萄糖转运蛋白异构体GLUT 2的表达，GLUT 2是胰腺β细胞葡萄糖感应机制的重要组成部分。在移植后12、36和60周获取带有移植物的肝脏，组织切片进行GLUT 2和主要胰岛肽的免疫过氧化物酶染色。在门静脉区和与肝细胞相邻处发现了不同大小的胰岛细胞聚集体。在所有时间点，移植物中的β细胞显示出与非糖尿病大鼠胰岛相当的GLUT 2表达。含有免疫反应性胰岛素和胰岛淀粉样多肽的胰岛细胞丰富，而这些移植物中胰高血糖素和生长抑素染色阳性的细胞稀少。结果表明，移植到肝脏中的胰岛β细胞尽管最初暴露于慢性高血糖环境，但仍有能力长期稳定表达GLUT 2。
