Takahashi N, Lee K B, Nakagawa H, Tsukamoto Y, Masuda K, Lee Y C
GlycoLab, Nakano Vinegar Co., Ltd., Handa City Aichi, Japan.
Anal Biochem. 1998 Jan 15;255(2):183-7. doi: 10.1006/abio.1997.2463.
We describe here the structures of several new N-glycan oligosaccharides from horseradish peroxidase (HRP). Glycopeptides from HRP were digested with glycoamidase A (from sweet almond) to release the N-glycans. The oligosaccharides were reductively aminated with 2-aminopyridine, and separated by high-performance liquid chromatography on octadecylsilyl- and amide-silica columns for two-dimensional mapping (Tomiya et al., Anal. Biochem. 171, 73-90, 1988). The N-glycans were also analyzed by electrospray ionization mass spectrometry. Two different lots of HRP showed a considerable difference in their N-glycan composition.
我们在此描述了几种来自辣根过氧化物酶（HRP）的新型N-聚糖寡糖的结构。HRP的糖肽用（来自甜杏仁的）糖酰胺酶A消化以释放N-聚糖。寡糖用2-氨基吡啶进行还原性胺化，并通过在十八烷基甲硅烷基柱和酰胺硅胶柱上的高效液相色谱进行分离以进行二维图谱分析（富宫等人，《分析生物化学》171, 73 - 90, 1988）。N-聚糖也通过电喷雾电离质谱进行分析。两批不同的HRP在其N-聚糖组成上显示出相当大的差异。
