Gao X P, Akhter S R, Rubinstein I
Department of Medicine, University of Illinois at Chicago, USA.
J Appl Physiol (1985). 1998 Jan;84(1):169-76. doi: 10.1152/jappl.1998.84.1.169.
The purpose of this study was to determine whether bradykinin mediates ovalbumin-induced increase in macromolecular efflux from the nasal mucosa of ovalbumin-sensitized hamsters in vivo and, if so, whether the L-arginine/nitric oxide biosynthetic pathway transduces, in part, this response. We found that suffusion of ovalbumin onto the in situ nasal mucosa of ovalbumin-sensitized hamsters, but not of controls, elicited a significant time- and concentration-dependent increase in clearance of fluorescein isothiocyanate-labeled dextran (mol mass, 70 kDa; P < 0.05). HOE-140, but not des-Arg9,[Leu8]-bradykinin, and NG-L-arginine methyl ester (L-NAME), but not NG-D-arginine methyl ester, significantly attenuated ovalbumin-induced responses. L-Arginine, but not D-arginine, abolished the effects of L-NAME. L-NAME also significantly attenuated bradykinin-, but not adenosine-induced increase in macromolecular efflux from the in situ nasal mucosa. Overall, these data suggest that ovalbumin increases macromolecular efflux from the in situ nasal mucosa of ovalbumin-sensitized hamsters, in part, by producing bradykinin with subsequent activation of the L-arginine/ nitric oxide biosynthetic pathway.
本研究的目的是确定缓激肽是否介导卵清蛋白诱导的卵清蛋白致敏仓鼠鼻黏膜中大分子物质外流增加，以及如果是这样，L-精氨酸/一氧化氮生物合成途径是否部分传导这种反应。我们发现，将卵清蛋白灌注到卵清蛋白致敏仓鼠的原位鼻黏膜上，而不是对照组的鼻黏膜上，会引起异硫氰酸荧光素标记的右旋糖酐（分子量70 kDa；P < 0.05）清除率显著的时间和浓度依赖性增加。HOE-140而非去-Arg9,[Leu8]-缓激肽，以及NG-L-精氨酸甲酯（L-NAME）而非NG-D-精氨酸甲酯，能显著减弱卵清蛋白诱导的反应。L-精氨酸而非D-精氨酸可消除L-NAME的作用。L-NAME也显著减弱缓激肽而非腺苷诱导的原位鼻黏膜大分子物质外流增加。总体而言，这些数据表明卵清蛋白通过产生缓激肽并随后激活L-精氨酸/一氧化氮生物合成途径，部分增加了卵清蛋白致敏仓鼠原位鼻黏膜的大分子物质外流。
