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[Optimization of the polymerase chain reaction (PCR) for detection and characterization of shigatoxin producing Escherichia coli (STEC) in food].

作者信息

Gallien P, Richter H, Much C, Fröbe I, Perlberg K W, Protz D

机构信息

Bundesinstitut für gesundheitlichen Verbraucherschutz und Veterinärmedizin, Standorte in Dessau.

出版信息

Berl Munch Tierarztl Wochenschr. 1997 Dec;110(11-12):422-6.

PMID:9451840
Abstract

The polymerase chain reaction (PCR) was improved to detect shigatoxin producing Escherichia coli (STEC) in milk. Numbers of colony forming units (cfu) in test samples, concentrations and types of primers, amount of MgCl2, types of thermostable DNA-Polymerase, and cycling programs were modified up to obtain the cleanest electrophoretic pattern and the highest sensitivity. Experimental conditions for further characterization of STEC-isolates by means of PCR are given by summarizing data from literature.

摘要

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